Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR.
نویسندگان
چکیده
Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 45 5 شماره
صفحات -
تاریخ انتشار 2007